Gibson and CU Researchers win National Science Foundation grant to study brain

CU Anschutz and CU Boulder scientists to use unique microscope for high risk brain research

AURORA, Colo. (August 18, 2016) – Researchers from the University of Colorado Anschutz Medical Campus and the University of Colorado Boulder have won a $800,000 grant from the National Science Foundation to try and reconnect neural communication between parts of the brain where it has been severed.

If successful, this could have major implications for those suffering brain injury, stroke, Parkinson’s disease and other neurological problems.

The team of neuroscientists and engineers will use a special lightweight microscope, which they designed, to peer into and control the living brain of a mouse as they try to reconnect parts of the brain that no longer communicate with each other.

The miniature microscope, using a unique electrowetting lens, is mounted on the head of a mouse and with its high-powered, fiber-optic light can actually view and control neural activity as it happens.

“Adaptive optical devices that are included in a miniature microscope are a game changer,” said grant co-investigators Juliet Gopinath, assistant professor in electrical, computer and energy engineering and Victor Bright, professor of mechanical engineering, both at CU Boulder. “They enable truly miniature 3D imaging devices without mechanically moving parts.”

According to Gopinath and Bright, the electrowetting lens is compact, low power and has good optical quality making it ideal for this kind of research. The liquid lens can change shape when voltage is applied.

The team will use an optic fiber to disrupt the signals between the olfactory bulb of a mouse, which receives information on odors, and the olfactory cortex, the part of the brain that allows it to smell. In essence, they will shut down its ability to smell and then try to restore it by activating the olfactory cortex using the miniature microscope.

The mouse will be awake and behaving normally throughout this while the team views and controls what is happening in the brain with the electrowetting fiber-coupled microscope. They can stimulate the animal’s brain activity using powerful laser light that flows through the microscope’s fiber-optic bundle.

“One major problem with the brain is that with certain diseases or injuries, one part of the brain stops talking to another,” said co-investigator Diego Restrepo, professor of cell and developmental biology and director of the Center for NeuroScience at the University of Colorado School of Medicine. “If someone has a stroke they may no longer be able to speak.”

Once connections between brain areas are lost, it is difficult to get them communicating again.

Restrepo said if researchers are successful reestablishing brain connections in a mouse, they may be able do the same in humans with brain injury or disease.

“For example, if there is loss of connection between the retina that detects the image in the eyes and the visual cortex, in the back of the brain the patient has a problem detecting images that in the worst case leads to blindness,” Restrepo said. “That loss of connection between the retina and visual cortex can be due to neural problems such as stroke, neuro-immune disease or traumatic brain injury.”

If this experiment is successful, he said, this microscope could eventually be modified to activate neurons in the visual cortex based on the visual input. In other words, creating a bridge between two parts of the brain where communication has stopped.

“This is an interdisciplinary grant which combines bioengineering with neurological applications,” said Emily Gibson, assistant professor of bioengineering at CU Anschutz. “The idea is to use this device which can image individual neurons and stimulate those individual neurons in that 3D volume.”

She also noted that two of the principal investigators on the grant are women, a rarity in the field of engineering.

“This particular grant is for high risk, high payoff approaches,” she said. “And this is a very high risk project. We are pushing the technology farther and seeing if we can use these optical tools to ultimately make an impact on humans.”

The grant is funded under a program from the National Science Foundation known as the “Integrative Strategies for Understanding Neural and Cognitive Systems (NSF-NCS).”

It is one element of NSF’s broader effort directed at Understanding the Brain, ( a multi-year activity that includes NSF’s participation in the Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative.

The team also won a second NSF grant of $200,000 to be used in the dissemination and commercialization of its microscope.

See the corresponding story in The Denver Post.

Karunanithi and team receives National Science Foundation Education Grant

Arun Karananithi 432
Arun Karunanithi Associate Professor, Civil Engineering

The Department of Civil Engineering and the Center for Sustainable Infrastructure System has received a NSF – IUSE grant (2 years; $ 249,967) titled “Sustainable Stem Learning Program (S2LP): Promoting systems thinking to aid holistic undergraduate education.” Arun Karunanithi (PI), will lead this project and work with Mike Tang (co-PI), Vivian Shyu (co-PI; Psychology), and Azadeh Bolhari toward development and teaching of two engineering undergraduate courses with an aim to understand if a new teaching approach based on concept mapping can aid in the development of systems thinking skills of undergraduate students.

Further, as part of this grant, systems thinking oriented teaching innovation is being incorporated in a graduate-level sustainability course. The PIs will develop new course material based on concept mapping for the three classes and assessment data collected from these courses will help us understand the effectiveness of the new teaching intervention towards development of students systems thinking skills. As part of this project, baseline data related to cognitive styles and systems thinking skills of undergraduate students of different majors is being collected through questionnaires and tests.

Gibson’s research featured in Denver Post

Gibson, EmilyEmily Gibson, assistant professor of bioengineering, and colleagues with the CU School of Medicine and CU Boulder have created a miniature, fiber-optic microscope that can look deep inside a living brain. Their work was featured in the Denver Post on May 12. The research was made possible by a $1M grant from the National Science Foundation and will also be featured on the NSF website.

Read the Denver Post story here.

Congratulations, Emily!


NSF CAREER Award supports work in smart polymers

Yakacki (9-12)-WEBAssistant Professor of Mechanical Engineering Christopher Yakacki received a 2014 National Science Foundation CAREER Award. Yakacki’s CAREER award project, “A Two-Stage Processing Approach to Shape-Switching Liquid-Crystalline Elastomers for Biomedical Applications,” is a five-year investigation into the development of a reaction mechanism to tailor and manufacture liquid-crystalline elastomers (LCEs) for biomedical applications.

LCEs are a class of smart polymers that can repeatedly change shape and optical properties in response to a stimulus, such as heat or light. Traditionally, LCEs have been difficult to synthesize and manufacture for applications such as biomedical devices. This CAREER award is to investigate a new approach and reaction mechanism to tailor and manufacture these materials for biomedical applications, specifically shape-changing biomedical devices.

Yakacki’s CAREER award will also serve to create summer workshops for local high school students, which will give them a hands-on experience in how smart polymers can be used in biomedical applications. Using this new technology, Yakacki will apply his teaching and industry experience to design and develop interference devices for anterior cruciate ligament (ACL) tears, which also illustrates how the fields of mechanical engineering, materials science, and bioengineering can combine. He hopes to show that engineering isn’t a confined area of study, and that although there are individual degree programs, engineers often solve problems using an interdisciplinary approach. Through this endeavor, Yakacki wants to give students a better look at how a college education can lead to unique, real-world opportunities and experiences.